Extraction Preparation and Anti-Wrinkle Biomarkers from Ilex x wandoensis C. F. Miller and M. Kim Hybrid nov., a New Plant

Ilex x wandoensis and M. Kim hybrid nov. (IW) is a new variety formed through the natural hybridization of Ilex. cornuta Thunb Iles. integra Thunb. It was first discovered on Wando Island, Korea. IW has not been studied to date; we investigated industrial application potential leaf for time. We prepared hot water solvent extracts tested biological activity. extract confirmed have antioxidant anti-wrinkle effects. The had high effect, hexane, ethyl acetate, acetone showed excellent elastase inhibitory HPLC GC-MS analyses β-amyrin erythrodiol identified them as biomarkers Here, report time active ingredients in leaf, representing plant variety, suggest that could be developed cosmetic material.